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IGG4 FITC, IFA

IGG4 FITC, IFA

₹3,491.00मूल्य

 

  • Overview

    USEFUL FOR 
     

    Confirming a diagnosis of pemphigoid, pemphigus, epidermolysis bullosa acquisita, or bullous lupus erythematosus

    METHOD NAME 
     

    Indirect Immunofluorescence

     

    REPORTING NAME 
     
    Cutaneous Immfluor. Ab, S (IgG)
    ALIASES 
     

    Basement Membrane Antibodies

    Bullous Pemphigoid

    Cicatricial Pemphigoid

    CII, Serum

    Circulating IgG Basement Membrane Zone Antibody

    Circulating Intercellular Ab Study

    Epidermal Cell Surface Antibody

    Epidermal Fluorescence Antibody

    Epidermolysis Bullosa Acquisita

    Epithelial Antibody

    Herpes gestationis

    IMF - Epidermal Fluorescent Ab

    Indirect Immunofluorescence

    Intercellular Substance Antibody

    Pemphigoid

    Pemphigus

    Skin Basement Membrane Antibodies

    Split-Skin Assay

    Lupus Erythematosus (LE)

    Bullous Lupus Erythematosus

  • Specimen

    SPECIMEN TYPE 
     

    Serum

    SPECIMEN REQUIRED 
     

    Collection Container/Tube:

    Preferred: Red top

    Acceptable: Serum gel

    Submission Container/Tube: Plastic vial

    Specimen Volume: 2 mL

    SPECIMEN MINIMUM VOLUME 
     

    0.5 mL

  • Clinical Info

    USEFUL FOR 
     

    Confirming a diagnosis of pemphigoid, pemphigus, epidermolysis bullosa acquisita, or bullous lupus erythematosus

    CLINICAL INFORMATION 
     

    IgG anti-basement membrane zone (BMZ) antibodies are produced by patients with pemphigoid. In most patients with bullous pemphigoid, serum contains IgG anti-BMZ antibodies, while in cicatricial pemphigoid circulating IgG anti-BMZ antibodies are found in a minority of cases. Sensitivity of detection of anti-BMZ antibodies is increased when serum is tested using sodium chloride-split primate skin as substrate.

     

    Circulating IgG anti-BMZ antibodies are also detected in patients with epidermolysis bullosa acquisita and bullous eruption of lupus erythematosus.

     

    IgG anti-cell surface (CS) antibodies are produced by patients with pemphigus. The titer of anti-CS antibodies generally correlates with disease activity of pemphigus.

    REFERENCE VALUES 
     

    Report includes presence and titer of circulating antibodies. If serum contains basement zone (BMZ) antibodies on split-skin substrate, patterns will be reported as:

    1) Epidermal pattern, consistent with pemphigoid

    2) Dermal pattern, consistent with epidermolysis bullosa acquisita

     

    Negative in normal individuals

    INTERPRETATION 
     

    Indirect immunofluorescence (IF) testing may be diagnostic when histologic or direct IF studies are only suggestive, nonspecific, or negative.

     

    Anti-cell surface (CS) antibodies correlate with a diagnosis of pemphigus.

     

    Anti-basement membrane zone (BMZ) antibodies correlate with a diagnosis of bullous pemphigoid, cicatricial pemphigoid, epidermolysis bullosa acquisita (EBA), or bullous eruption of lupus erythematosus (LE).

     

    If serum contains anti-BMZ antibodies, the pattern of fluorescence on sodium chloride (NaCl)-split skin substrate helps distinguish pemphigoid from EBA and bullous LE. Staining of the roof (epidermal side) or both epidermal and dermal sides of NaCl-split skin correlates with the diagnosis of pemphigoid, while fluorescence localized only to the dermal side of the split-skin substrate correlates with either EBA or bullous LE.

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